# Question: GTEx v10 smallRNA expression read counts **URL:** \ **Category:** Help\ **Created:** [February 14, 2025, 2:57pm UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263 "2025-02-14T14:57:25Z")\ **Posts on this page:** 7\ **Page:** 1
**Author:** ![farzamani](https://avatars.discourse-cdn.com/v4/letter/f/df705f/32.png) [@farzamani](https://help.anvilproject.org/u/farzamani)\ **Post date:** [February 14, 2025, 2:57pm UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/1 "2025-02-14T14:57:25Z")
Hi, The improvement from GTEx v8 is really impressive and I believe a lot of people will benefit from it. I have a question regarding the data for small RNA-seq (this one: GTEx\_Analysis\_v10\_smallRNA\_gene\_reads.txt.gz). The data doesn’t seem to be a raw count (I assume) and there are some negative values as well in the table. What would that be, if some normalization was performed? Thanks! Best, Farhad ---
**Author:** ![Javier-CP](https://yyz1.discourse-cdn.com/flex027/user_avatar/help.anvilproject.org/javier-cp/32/168_2.png) [@Javier-CP](https://help.anvilproject.org/u/Javier-CP)\ **Post date:** [February 18, 2025, 5:28pm UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/2 "2025-02-18T17:28:23Z")
Hello @farzamani, Thank you for your question! I opened a ticket with the GTEx portal to ask for some clarification here. Will get back to you as soon as we hear from them. Javier ---
**Author:** ![farzamani](https://avatars.discourse-cdn.com/v4/letter/f/df705f/32.png) [@farzamani](https://help.anvilproject.org/u/farzamani)\ **Post date:** [March 11, 2025, 9:01am UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/3 "2025-03-11T09:01:13Z")
Hi Javier, thanks for your help! ---
**Author:** ![Ignas](https://avatars.discourse-cdn.com/v4/letter/i/e495f1/32.png) [@Ignas](https://help.anvilproject.org/u/Ignas)\ **Post date:** [April 9, 2025, 12:38pm UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/4 "2025-04-09T12:38:38Z")
Hello, I just wanted to ask if there’s been any update on this topic? I was having the same question as Farhad would be really thankful for any explanation about how to interpret the numbers in the smallRNA reads file. Thanks in advance. Ignas ---
**Author:** ![farzamani](https://avatars.discourse-cdn.com/v4/letter/f/df705f/32.png) [@farzamani](https://help.anvilproject.org/u/farzamani)\ **Post date:** [May 8, 2025, 10:41am UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/5 "2025-05-08T10:41:07Z")
Hi @Ignas, If you haven’t checked in the portal, they have now updated the smallRNAseq data, including the metadata and annotation. Best, Farhad ---
**Author:** ![Javier-CP](https://yyz1.discourse-cdn.com/flex027/user_avatar/help.anvilproject.org/javier-cp/32/168_2.png) [@Javier-CP](https://help.anvilproject.org/u/Javier-CP)\ **Post date:** [May 8, 2025, 1:34pm UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/6 "2025-05-08T13:34:06Z")
Thank you @farzamani for the update! Attaching the link to the documentation: - [https://storage.googleapis.com/adult-gtex/bulk-gex/v10/small-RNA/README.pdf](https://storage.googleapis.com/adult-gtex/bulk-gex/v10/small-RNA/README.pdf) Best, ---
**Author:** ![Javier-CP](https://yyz1.discourse-cdn.com/flex027/user_avatar/help.anvilproject.org/javier-cp/32/168_2.png) [@Javier-CP](https://help.anvilproject.org/u/Javier-CP)\ **Post date:** [May 8, 2025, 1:34pm UTC](https://help.anvilproject.org/t/question-gtex-v10-smallrna-expression-read-counts/263/7 "2025-05-08T13:34:34Z")